As of the U.S. Census on April 1, 2000, Bartlett, with a population of 40,543, was the 12th largest city in Tennessee (2nd in Shelby County, after Memphis).

The ranking is as follows (with Shelby County cities in Blue):

Rank	City	Population
1	Memphis	650,100
2	Nashville-Davidson	569,891
3	Knoxville	173,890
4	Chattanooga	155,554
5	Clarksville	103,455
6	Murfreesboro	68,816
7	Jackson	59,643
8	Johnson City	55,469
9	Kingsport	44,905
10	Franklin	41,842
11	Hendersonville	40,620
12	Bartlett	40,543
13	Germantown	37,348
14	Cleveland	37,192
15	Columbia	33,055
16	Collierville	31,872
	Millington	10,433
	Lakeland	6,862
	Arlington	2,569